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Detecting miRNA Biomarkers from Extracellular Vesicles for Cardiovascular Disease with a Microfluidic System

Abstract

According to World Health Organization reports, cardiovascular diseases (CVDs) are amongst the major causes of death globally and are responsible for over 18 million deaths every year. Traditional detection methods for CVDs include cardiac computerized tomography scans, electrocardiography, and myocardial perfusion imaging scans. Although diagnosis of CVDs through such bio-imaging techniques is common, these methods are relatively costly and cannot detect CVDs in their earliest stages. In contrast, levels of certain micro RNAs (miRNAs) biomarkers extracted from extracellular vesicles (EVs) in the bloodstream have been recognized as promising indicators for early CVDs detection . However, detection and quantification of miRNA using existing methods are relatively labor-intensive and time-consuming. In this study, a new integrated microfluidic system equipped with highly sensitive field-effect transistors (FET) was capable of performing EV extraction, EV lysis, target miRNA extraction and miRNA detection within 5 hrs. The limit of detection was within physiological range (femtomolar) for two targeted miRNAs, miR-21 and miR-126, meaning that this integrated microfluidic system has the potential to be used as a tool for early detection of CVDs.

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Publication details

The article was received on 13 Apr 2018, accepted on 06 Aug 2018 and first published on 08 Aug 2018


Article type: Paper
DOI: 10.1039/C8LC00386F
Citation: Lab Chip, 2018, Accepted Manuscript
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    Detecting miRNA Biomarkers from Extracellular Vesicles for Cardiovascular Disease with a Microfluidic System

    H. Cheng, C. Fu, W. Kuo, Y. Chen, Y. Chen, Y. Lee, K. Li, C. Chen, H. Ma, P. Huang, Y. Wang and G. Lee, Lab Chip, 2018, Accepted Manuscript , DOI: 10.1039/C8LC00386F

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