Jump to main content
Jump to site search

Issue 10, 2018
Previous Article Next Article

“Dip-and-read” paper-based analytical devices using distance-based detection with color screening

Author affiliations


An improved paper-based analytical device (PAD) using color screening to enhance device performance is described. Current detection methods for PADs relying on the distance-based signalling motif can be slow due to the assay time being limited by capillary flow rates that wick fluid through the detection zone. For traditional distance-based detection motifs, analysis can take up to 45 min for a channel length of 5 cm. By using a color screening method, quantification with a distance-based PAD can be achieved in minutes through a “dip-and-read” approach. A colorimetric indicator line deposited onto a paper substrate using inkjet-printing undergoes a concentration-dependent colorimetric response for a given analyte. This color intensity-based response has been converted to a distance-based signal by overlaying a color filter with a continuous color intensity gradient matching the color of the developed indicator line. As a proof-of-concept, Ni quantification in welding fume was performed as a model assay. The results of multiple independent user testing gave mean absolute percentage error and average relative standard deviations of 10.5% and 11.2% respectively, which were an improvement over analysis based on simple visual color comparison with a read guide (12.2%, 14.9%). In addition to the analytical performance comparison, an interference study and a shelf life investigation were performed to further demonstrate practical utility. The developed system demonstrates an alternative detection approach for distance-based PADs enabling fast (∼10 min), quantitative, and straightforward assays.

Graphical abstract: “Dip-and-read” paper-based analytical devices using distance-based detection with color screening

Back to tab navigation

Supplementary files

Publication details

The article was received on 09 Feb 2018, accepted on 29 Mar 2018 and first published on 05 Apr 2018

Article type: Paper
DOI: 10.1039/C8LC00168E
Citation: Lab Chip, 2018,18, 1485-1493

  •   Request permissions

    “Dip-and-read” paper-based analytical devices using distance-based detection with color screening

    K. Yamada, D. Citterio and C. S. Henry, Lab Chip, 2018, 18, 1485
    DOI: 10.1039/C8LC00168E

Search articles by author