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Issue 1, 2018
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Micro free flow electrophoresis

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Micro free-flow electrophoresis (μFFE) is a continuous separation technique in which analytes are streamed through a perpendicularly applied electric field in a planar separation channel. Analyte streams are deflected laterally based on their electrophoretic mobilities as they flow through the separation channel. A number of μFFE separation modes have been demonstrated, including free zone (FZ), micellar electrokinetic chromatography (MEKC), isoelectric focusing (IEF) and isotachophoresis (ITP). Approximately 60 articles have been published since the first μFFE device was fabricated in 1994. We anticipate that recent advances in device design, detection, and fabrication, will allow μFFE to be applied to a much wider range of applications. Applications particularly well suited for μFFE analysis include continuous, real time monitoring and microscale purifications.

Graphical abstract: Micro free flow electrophoresis

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The article was received on 19 Jun 2017, accepted on 16 Oct 2017 and first published on 17 Oct 2017

Article type: Critical Review
DOI: 10.1039/C7LC01105A
Lab Chip, 2018,18, 27-40

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    Micro free flow electrophoresis

    A. C. Johnson and M. T. Bowser, Lab Chip, 2018, 18, 27
    DOI: 10.1039/C7LC01105A

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