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Issue 4, 2018
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High-throughput sorting of eggs for synchronization of C. elegans in a microfluidic spiral chip

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Abstract

In this study, we report the use of a high-throughput microfluidic spiral chip to screen out eggs from a mixed age nematode population, which can subsequently be cultured to a desired developmental stage. For the sorting of a mixture containing three different developmental stages, eggs, L1 and L4, we utilized a microfluidic spiral chip with a trapezoidal channel to obtain a sorting efficiency of above 97% and a sample purity (SP) of above 80% for eggs at different flow rates up to 10 mL min−1. The result demonstrated a cost-effective, simple, and highly efficient method for synchronizing C. elegans at a high throughput (∼4200 organisms per min at 6 mL min−1), while eliminating challenges such as clogging and non-reusability of membrane-based filtration. Due to its simplicity, our method can be easily adopted in the C. elegans research community.

Graphical abstract: High-throughput sorting of eggs for synchronization of C. elegans in a microfluidic spiral chip

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Article information


Submitted
15 Sep 2017
Accepted
13 Jan 2018
First published
17 Jan 2018

Lab Chip, 2018,18, 679-687
Article type
Paper

High-throughput sorting of eggs for synchronization of C. elegans in a microfluidic spiral chip

S. Sofela, S. Sahloul, M. Rafeie, T. Kwon, J. Han, M. E. Warkiani and Y. Song, Lab Chip, 2018, 18, 679
DOI: 10.1039/C7LC00998D

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