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Internal standard evaluation for bioimaging soybean leaves through laser ablation inductively coupled plasma mass spectrometry: a plant nanotechnology approach

Abstract

This work evaluates the use of internal standard for acquiring elemental mapping of 107Ag, 55Mn and 63Cu using laser ablation inductively couple plasma mass spectrometry in the imaging mode from soybean leaves cultivated in the absence or in the presence of silver nanoparticles (40 nm as average size), and also using silver nitrate for comparative purposes. Carbon, which is naturally present in plants and currently used as internal standard for plant analysis is evaluated as 12C and 13C, as well as 28Si and 31P, as viable alternative. The optimized conditions are established using the certified reference material – CRM 1573a Tomato leaves, considering those parameters involved in the plasma production (RF power – 1000 W, Carrier and auxiliary gas flow rates – 1.1 L min-1 and 1.8 L min-1, respectively) as well as in the laser ablation process (laser intensity – 55%, frequency – 20 Hz, spot size diameter – 80 μm, and scan speed – 70 μm s-1). Additionally, the choice of the correct internal standard (13C+) is made based on the highest precision obtained (RSD of 37, 15, and 4% for 107Ag+, 55Mn+, and 63Cu+, respectively) as well as the highest homogeneity in the samples surface (verified through the images). The analysis of the images reveals not only the basal level of Ag present in the leaves, indicating its poor translocation, but also the homeostasis of Mn and Cu highly affected in plants cultivated in the presence of silver nitrate than silver nanoparticles. The distribution of Mn and Cu in the leaves is similar when compared the control plants and those cultivated in the presence of nanoparticles.

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Publication details

The article was accepted on 07 Aug 2018 and first published on 07 Aug 2018


Article type: Paper
DOI: 10.1039/C8JA00254A
Citation: J. Anal. At. Spectrom., 2018, Accepted Manuscript
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    Internal standard evaluation for bioimaging soybean leaves through laser ablation inductively coupled plasma mass spectrometry: a plant nanotechnology approach

    K. C. Madrid and M. A. Zezzi Arruda, J. Anal. At. Spectrom., 2018, Accepted Manuscript , DOI: 10.1039/C8JA00254A

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