Jump to main content
Jump to site search


A visual electrochemiluminescence resonance energy transfer/surface plasmon coupled electrochemiluminescence nanosensor for Shiga toxin-producing Escherichia coli detection

Author affiliations

Abstract

In this work, we used a microwave method to synthesize green, pollution-free BN quantum dots (QDs). In order to explore new high-performance electrochemiluminescence (ECL) sensors, a BN QDs-based ECL biosensor was established with an ECL resonance energy transfer (ECL-RET)/surface plasmon coupled ECL (SPC-ECL) sensing mode in this work. Firstly, BN QDs and Au NPs were bound to the ends of hairpin DNA, respectively. Therefore, the Au NPs effectively quenched the ECL signal of the BN QDs due to ECL-RET. When hairpin DNA identified the target DNA, the hairpin structure transformed to a linear conformation, and the distance between BN QDs and Au NPs enlarged. As a result, the SPC-ECL effect took the place of ECL-RET. The ECL signal was enhanced, by about six times higher than BN QDs-Au NPs-hairpin DNA conjugates. The new ECL-RET/SPC-ECL sensing mode greatly improved the sensitivity and accuracy of the QDs-based ECL sensor. The proposed biosensor could accurately quantify the Shiga toxin-producing Escherichia coli (STEC) gene from 1 pmol L−1 to 5 nmol L−1 with a limit of detection (LOD) of 0.3 pmol L−1. The new ECL biosensor showed excellent selectivity, stability and high sensitivity, and it was applied in spiked human serum samples with satisfactory results. Moreover, the change of the BN QDs ECL signal could be easily observed with the help of a smartphone camera. Benefiting from the ECL-RET/SPC-ECL sensing mode, to the best of our knowledge, this is the first time that visual QDs ECL signals were directly observed by a household smartphone CMOS.

Graphical abstract: A visual electrochemiluminescence resonance energy transfer/surface plasmon coupled electrochemiluminescence nanosensor for Shiga toxin-producing Escherichia coli detection

Back to tab navigation

Supplementary files

Publication details

The article was received on 25 Sep 2018, accepted on 08 Nov 2018 and first published on 08 Nov 2018


Article type: Paper
DOI: 10.1039/C8GC03010C
Citation: Green Chem., 2018, Advance Article
  •   Request permissions

    A visual electrochemiluminescence resonance energy transfer/surface plasmon coupled electrochemiluminescence nanosensor for Shiga toxin-producing Escherichia coli detection

    Y. Liu, X. Chen, M. Wang and Q. Ma, Green Chem., 2018, Advance Article , DOI: 10.1039/C8GC03010C

Search articles by author

Spotlight

Advertisements