An integrated process for enzymatic catalysis allowing product recovery and enzyme reuse by applying thermoreversible aqueous biphasic systems

Ana M. Ferreira; Helena Passos; Akiyoshi Okafuji; Ana P. M. Tavares; Hiroyuki Ohno; Mara G. Freire; João A. P. Coutinho

doi:10.1039/C7GC03880A

An integrated process for enzymatic catalysis allowing product recovery and enzyme reuse by applying thermoreversible aqueous biphasic systems

Ana M. Ferreira,^a Helena Passos,^a Akiyoshi Okafuji,^b Ana P. M. Tavares,^a Hiroyuki Ohno,^b Mara G. Freire*^a and João A. P. Coutinho*^a

Author affiliations

* Corresponding authors

^a CICECO – Aveiro Institute of Materials, Chemistry Department, University of Aveiro, 3810-193 Aveiro, Portugal
E-mail: maragfreire@ua.pt, jcoutinho@ua.pt

^b Department of Biotechnology and Life Science, Tokyo University of Agriculture and Technology, Koganei, Japan

Abstract

Herein, thermoreversible aqueous biphasic systems (ABS) composed of ammonium-based zwitterions (ZIs) and polymers are disclosed to act as integrated bioreaction–separation processes. The biocatalytic reaction involving laccase occurs in homogeneous media, after which small changes in temperature induce the formation of two phases and the complete separation of the enzyme from the products in a single-step. These systems also allow the recovery and reuse of the enzyme, along with the ZI-rich phase, contributing towards the development of sustainable biocatalytic processes.

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Publication details

The article was received on 28 Dec 2017, accepted on 15 Feb 2018 and first published on 15 Feb 2018

Article type: Communication

DOI: 10.1039/C7GC03880A

Citation: Green Chem., 2018,20, 1218-1223

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An integrated process for enzymatic catalysis allowing product recovery and enzyme reuse by applying thermoreversible aqueous biphasic systems

A. M. Ferreira, H. Passos, A. Okafuji, A. P. M. Tavares, H. Ohno, M. G. Freire and J. A. P. Coutinho, Green Chem., 2018, 20, 1218
DOI: 10.1039/C7GC03880A
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Green Chemistry

An integrated process for enzymatic catalysis allowing product recovery and enzyme reuse by applying thermoreversible aqueous biphasic systems

Abstract

Supplementary files

Publication details

An integrated process for enzymatic catalysis allowing product recovery and enzyme reuse by applying thermoreversible aqueous biphasic systems

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