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Simultaneous extraction and enrichment of polyphenol and lutein from marigold (Tagetes erecta L.) flower by enzyme-assisted ethanol/ammonium sulfate system

Abstract

Enzyme-assisted aqueous two-phase extraction (EA-ATPE) using ethanol/ammonium sulfate system was investigated for total polyphenol (TP) and lutein from marigold flower. The important factors were investigated by single factor experiment and response surface methodology combined with Box-Behnken Design to optimize the operating parameters of EA-ATPE, and the maximum yields of TP and lutein were 83.56±0.69 mg/g and 5.59±0.13 mg/g. Compared with aqueous two-phase extraction and soxhlet extraction (SE), the extraction yield of TP by EA-ATPE is 64.91% higher than that of SE, and the extract of EA-ATPE has a better antioxidant activity. The pretreatment effect was also researched by scanning electron microscope. Thus, EA-ATPE was an efficient method for extracting bioactive components from plants.

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Publication details

The article was received on 25 Sep 2018, accepted on 03 Dec 2018 and first published on 04 Dec 2018


Article type: Paper
DOI: 10.1039/C8FO01865K
Citation: Food Funct., 2018, Accepted Manuscript
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    Simultaneous extraction and enrichment of polyphenol and lutein from marigold (Tagetes erecta L.) flower by enzyme-assisted ethanol/ammonium sulfate system

    X. Fu, G. Zhang, L. Deng and Y. Dang, Food Funct., 2018, Accepted Manuscript , DOI: 10.1039/C8FO01865K

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