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Anti-hyperuricemic and nephroprotective effects of extracts from Chaenomeles sinensis (Thouin) Koehne in hyperuricemic mice

Abstract

The use of Chaenomeles sinensis (Thouin) Koehne (C. sinensis) was suggested for the treatment of hyperuricemia and gout in clinical experience. However, the exact mechanism of action is still unknown. In a present study, ethyl acetate fraction of C. sinensis fruit extract (CSF-E) was separated. Potassium oxonate (PO)-induced hyperuricemicmice and normal mice were administered with CSF-E at 60, 120 and 180 mg/kg, respectively for 7 days. Serum uric acid, creatinine and BUN levels, liver oxidative damage, as well as serum and hepatic XOD activities were primarily measured using kits assays. The evaluation of its nephroprotective effects was carried out by renal histopathological analysis. Simultaneously, renal protein levels of organic anion transporters, such as mURAT1 and mOAT1, were detected using western blotting to elucidate possible mechanisms. The results showed that CSF-E could significantly inhibit XOD activities in both serum and liver (p < 0.05), decreasing uric acid, creatinine and BUN levels in serum, and increasing levels in the excretion of uric acid by down-regulated of mURAT1 and up-regulated mOAT1 protein expression of kidney in hyperuricemic mice. Moreover, PO-induced alterations on levels of MDA, hepatic SOD and GSH-Px activities, as well as renal inflammation damages in hyperuricemic mice were effectively recovered by CSF-E at 120mg/kg. CSF-E possessed anti-hyperuricemic and nephroprotective effects by suppressing XOD activity, improving renal function and regulating renal mURAT1 and mOAT1 protein expression, which resulted in their beneficial effects of hyperuricemia and gout prevention.

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Publication details

The article was received on 23 Jul 2018, accepted on 24 Sep 2018 and first published on 25 Sep 2018


Article type: Paper
DOI: 10.1039/C8FO01480A
Citation: Food Funct., 2018, Accepted Manuscript
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    Anti-hyperuricemic and nephroprotective effects of extracts from Chaenomeles sinensis (Thouin) Koehne in hyperuricemic mice

    R. Zhang, S. Zhan, S. Li, Z. Zhu, J. He, J. M. Lorenzo and F. J. Barba, Food Funct., 2018, Accepted Manuscript , DOI: 10.1039/C8FO01480A

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