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Narrow-leafed lupin (Lupinus angustifolius L.) seed β-conglutins reverse back the induced insulin resistance in pancreatic cells

Abstract

Insulin resistance (IR) is the main contributing problem leading to the development of the type 2 diabetes. In this study, we have purified recombinant β-conglutin proteins (rβ1 to rβ4, and rβ6) from narrow-leafed lupin (NLL) by using affinity-chromatography. The objective of this study was to evaluate the capacity of these β-conglutins to improve IR state using ex vivo and in vitro systems. rβ1, rβ3, and rβ6 produced lower levels of pro-inflammatory mediator nitric oxide (~ -7-fold in all cases); up-regulated mRNA expression levels of IRS-1 (+201, +173, +192%) and Glut-4 (+286, +121, +147%); increased levels of p85-PI3K (+188, +187, +137-fold) and Glut-4 (+503, +548, +515-fold) proteins; higher levels of phosphorylation of insulin signalling pathway activator p-IRS-1, and downstream mediators as p-Akt, p-Cbl, and p-Caveolin; as well as improved glucose uptake in insulin resistant (IR-C) culture cells. β-conglutin proteins were able to suppressing the oxidative stress produced by insulin induced resistance on PANC-1 control (C) cells by strongly reducing proteins oxidative carbonylation induced by ROS, and balancing the metabolic homeostasis in IR-C cells through regulation of mRNA expression. At the same time, β-conglutins are able to reduce the levels of pro-inflammatory mediator nitric oxide; and promoting anti-oxidative capacity of cells by increasing the levels of reduced glutathione. These results suggest NLL β-conglutins might play a fundamental role as functional food components, since β-conglutins nutraceutical properties could enhance the effectiveness of dietary improvement of type 2 diabetes complications.

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Publication details

The article was received on 11 Jun 2018, accepted on 06 Sep 2018 and first published on 10 Sep 2018


Article type: Paper
DOI: 10.1039/C8FO01164H
Citation: Food Funct., 2018, Accepted Manuscript
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    Narrow-leafed lupin (Lupinus angustifolius L.) seed β-conglutins reverse back the induced insulin resistance in pancreatic cells

    E. Lima-Cabello, S. Morales-Santana, J. León, V. Alché, A. Clemente, J. de Dios Alche and J. C. Jimenez-Lopez, Food Funct., 2018, Accepted Manuscript , DOI: 10.1039/C8FO01164H

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