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Issue 9, 2018
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Proanthocyanidin B2 attenuates postprandial blood glucose and its inhibitory effect on alpha-glucosidase: analysis by kinetics, fluorescence spectroscopy, atomic force microscopy and molecular docking

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Abstract

As a dimer of proanthocyanidin, proanthocyanidin B2 (PB2) was found to effectively attenuate postprandial blood glucose in mice after sucrose loading. Further studies showed that PB2 revealed strong inhibitory activity against α-glucosidase with an IC50 value of (0.23 ± 0.01) μg mL−1, and this process was reversible with a mixed-type inhibitory manner. PB2 quenched the intrinsic fluorescence of α-glucosidase through a static quenching mechanism and changed the micro-environments and conformations of α-glucosidase that led to an aggregation phenomenon. A further molecular docking study provided more information about the interactions of PB2 with the amino acid residues of α-glucosidase. Our results suggest that PB2 is useful for protection against hyperglycemia through inhibiting the activity of α-glucosidase.

Graphical abstract: Proanthocyanidin B2 attenuates postprandial blood glucose and its inhibitory effect on alpha-glucosidase: analysis by kinetics, fluorescence spectroscopy, atomic force microscopy and molecular docking

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Publication details

The article was received on 19 May 2018, accepted on 24 Aug 2018 and first published on 04 Sep 2018


Article type: Paper
DOI: 10.1039/C8FO00993G
Citation: Food Funct., 2018,9, 4673-4682

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    Proanthocyanidin B2 attenuates postprandial blood glucose and its inhibitory effect on alpha-glucosidase: analysis by kinetics, fluorescence spectroscopy, atomic force microscopy and molecular docking

    L. Han, L. Zhang, W. Ma, D. Li, R. Shi and M. Wang, Food Funct., 2018, 9, 4673
    DOI: 10.1039/C8FO00993G

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