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Transcriptome analysis reveals the mechanism of the effect of flower tea Coreopsis tinctoria on hepatic insulin resistance

Abstract

Non-Camellia tea and herbal medicine help prevent the development of diabetes and other metabolic diseases. Previous studies found that Coreopsis tinctoria (CT) flower tea increases insulin sensitivity and, in some high-fat diet (HFD)-fed rats, even prevents hepatic metabolic disorders. However, the molecular mechanisms by which CT improves insulin resistance are unknown. In this study, six-week-old rats were fed a normal diet (ND), HFD or HFD supplemented with CT for 8 weeks. Serum samples were collected, and the liver was extracted for RNA-seq gene expression analysis. Real-time PCR and Western blotting further verified the RNA-seq results. In our results, dietary CT ameliorated HFD-induced hepatosteatosis, glucose intolerance, and insulin resistance. In the HFD group, 1667 differentially expressed genes (DEGs) were identified compared with the ND group. In the CT group, 327 DEGs were identified compared with the HFD group. Some of these DEGs were related to insulin signalling, hepatic lipogenesis and glucose homeostasis. This study suggested that insulin resistance with hyperinsulinaemia, not insulin insufficiency, is an early problem in HFD-fed rats, and CT downregulates insulin secretion genes (e.g., Rasd1, Stxbp1 and Sfxn1). Hepatic gene and protein expression analyses indicated that the regulatory effects of CT on glucose and lipid homeostasis are likely mediated via the Akt/FoxO1 signalling pathway and regulated by the transcription factors HES1 and SHP. Our study provides transcriptomic evidence of the complex pathogenic mechanism involved in hepatic insulin resistance and that supplementation with CT improves insulin resistance at a global scale.

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Publication details

The article was received on 16 May 2018, accepted on 03 Oct 2018 and first published on 04 Oct 2018


Article type: Paper
DOI: 10.1039/C8FO00965A
Citation: Food Funct., 2018, Accepted Manuscript
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    Transcriptome analysis reveals the mechanism of the effect of flower tea Coreopsis tinctoria on hepatic insulin resistance

    B. Jiang, Q. Lv, W. Wan, K. Hu, L. Le, L. Xu and P. Xiao, Food Funct., 2018, Accepted Manuscript , DOI: 10.1039/C8FO00965A

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