Jump to main content
Jump to site search


A glucogalactomannan isolated from Agaricus Bisporus induces apoptosis in macrophages through the JNK/Bim/Caspase 3 pathway

Abstract

Agaricus bisporus is one of the most important edible and medicinal mushrooms in the world. It has been well known that Agaricus bisporus has an immunoregulatory role, but its active ingredients have not been completely identified. In this study, a glucogalactomannan named as TJ3, was isolated and purified from Agaricus bisporus. TJ3 (827 KDa) is composed of mannose, galactose, glucose and xylose in the ratio 28.26:27.82:20.88:9.87 mainly joined by β-linkages. Functional analysis of TJ3 revealed that it effectively induced apoptosis in RAW264.7 cells, a mouse macrophage cell line. Cell apoptosis was determined by an Annexin V/PI staining assay. After treatment with TJ3 (2 µg/mL) for 16 hr, apoptosis was observed in 34% of Raw cells (9% in non-treated control cells). TJ3 treatment remarkably increased the production of cleaved caspase-3, PARP and Bim, and decreased the level of Bcl-2 although no obvious change in the level of Bax was observed. Interestingly, further elucidation of the molecular mechanism underlying the role of TJ3 in induction of apoptosis showed that TJ3 activated the JNK signaling pathway through TLR4 and subsequently promoted the expression of Bim and activation of caspase-3. Our results demonstrate that TJ3 may be a novel active component in Agaricus bisporus responsible for its immunoregulatory role by induction of macrophage apoptosis.

Back to tab navigation

Publication details

The article was received on 14 May 2018, accepted on 09 Aug 2018 and first published on 10 Aug 2018


Article type: Paper
DOI: 10.1039/C8FO00944A
Citation: Food Funct., 2018, Accepted Manuscript
  •   Request permissions

    A glucogalactomannan isolated from Agaricus Bisporus induces apoptosis in macrophages through the JNK/Bim/Caspase 3 pathway

    X. zhao, L. Fang, D. Liu, C. Lai, Y. zhang, A. Zhou and J. Xie, Food Funct., 2018, Accepted Manuscript , DOI: 10.1039/C8FO00944A

Search articles by author

Spotlight

Advertisements