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In vitro digestion profile and calcium absorption studies of a sea cucumber ovum derived heptapeptide–calcium complex

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Abstract

In this study, a novel calcium-binding heptapeptide (NDEELNK) that is released during the trypsin hydrolysis of sea cucumber ovum was identified by peptidomics. The calcium binding mode, in vitro digestion profile and calcium absorption of the NDEELNK–calcium complex were investigated. The NDEELNK peptide could spontaneously bind calcium with a 1 : 1 stoichiometry, and the calcium-binding site might involve the carboxyl oxygen and amino nitrogen atoms of two glutamic acid and one aspartic acid residues in the NDEELNK peptide. The NDEELNK–calcium complex underwent disaggregation and self-aggregation in a mesh of smaller size during simulated gastrointestinal digestion, clarified by dynamic light scattering and confocal laser-scanning microscopy. In addition, the NDEELNK–calcium complex could be conducive to calcium absorption across Caco-2 cell monolayers. The findings from this research suggest possible utilization of hydrolyzed peptides from sea cucumber ovum as dietary supplements to improve calcium absorption.

Graphical abstract: In vitro digestion profile and calcium absorption studies of a sea cucumber ovum derived heptapeptide–calcium complex

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Publication details

The article was received on 09 May 2018, accepted on 19 Jul 2018 and first published on 25 Jul 2018


Article type: Paper
DOI: 10.1039/C8FO00910D
Citation: Food Funct., 2018, Advance Article
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    In vitro digestion profile and calcium absorption studies of a sea cucumber ovum derived heptapeptide–calcium complex

    P. Cui, S. Lin, Z. Jin, B. Zhu, L. Song and N. Sun, Food Funct., 2018, Advance Article , DOI: 10.1039/C8FO00910D

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