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Application of novel Solid Phase Extraction-NMR protocols for metabolic profiling of human urine

Abstract

Metabolite identification and annotation procedures are necessary for the discovery of biomarkers indicative of phenotypes or disease states, but these processes can be bottlenecked by the sheer complexity of biofluids containing thousands of different compounds. Here we describe low-cost novel SPE-NMR protocols utilising different cartridges and conditions, on both natural and artifical urine mixtures, which produce unique retention profiles useful to metabolic profiling. We find that different SPE methods applied to biofluids such as urine can be used to selectively retain metabolites based on compound taxonomy or other key functional groups, reducing peak overlap through concentration and fractionation of unknowns and hence promising greater control over the metabolite annotation/identification process.

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Publication details

The article was accepted on 06 Dec 2018 and first published on 21 Jan 2019


Article type: Paper
DOI: 10.1039/C8FD00220G
Citation: Faraday Discuss., 2018, Accepted Manuscript
  • Open access: Creative Commons BY license
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    Application of novel Solid Phase Extraction-NMR protocols for metabolic profiling of human urine

    D. McGill, E. Chekmeneva, J. C. Lindon, Z. Takats and J. K. Nicholson, Faraday Discuss., 2018, Accepted Manuscript , DOI: 10.1039/C8FD00220G

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