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In vitro characterization of reactive oxygen species (ROS) generation by the commercially available Mesosilver™ dietary supplement

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Abstract

Silver nanoparticles (AgNPs) are frequently orally consumed as dietary supplements with various commercially available products (e.g., Nano-silver™ and Mesosilver™) claimed to yield health benefits across an array of areas. While the mechanism by which AgNPs might provide health benefits is unclear, these benefits could potentially be related to either the release of dissolved monovalent Ag species (such as Ag+) or the ability of AgNPs to generate reactive oxygen species (ROS) and subsequent influence on the gut microbiome or eukaryotic cells. In this study, we investigate the in vitro Ag(I) and ROS (particularly hydrogen peroxide (H2O2) and hydroxyl radicals (˙OH)) generation by Mesosilver™ over a range of pHs (2–7) typical of the human gut and upper digestive system. Our results show that Mesosilver™ undergoes rapid oxygen-mediated oxidative dissolution and associated H2O2 production with the generation rate of both Ag(I) and H2O2 increasing with decrease in pH and increase in NaCl concentration. No impact of increase in temperature from 22 °C to 37 °C was observed on the oxidative dissolution of Mesosilver™. Addition of H2O2 further accelerates the dissolution of Mesosilver™ and causes production of ˙OH under all pH conditions investigated here. The presence of pepsin, an enzyme present in gastric fluid, resulted in a slight decrease in the extent of release of Ag(I) and H2O2 generation under acidic conditions. A kinetic model is developed based on our experimental results enabling prediction of the dissolved Ag(I) and ROS yield on dissolution of these nanoparticles under conditions typical of the human digestive system.

Graphical abstract: In vitro characterization of reactive oxygen species (ROS) generation by the commercially available Mesosilver™ dietary supplement

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Publication details

The article was received on 29 Jun 2018, accepted on 04 Oct 2018 and first published on 05 Oct 2018


Article type: Paper
DOI: 10.1039/C8EN00701B
Citation: Environ. Sci.: Nano, 2018, Advance Article
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    In vitro characterization of reactive oxygen species (ROS) generation by the commercially available Mesosilver™ dietary supplement

    H. Rong, S. Garg, P. Westerhoff and T. D. Waite, Environ. Sci.: Nano, 2018, Advance Article , DOI: 10.1039/C8EN00701B

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