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Issue 5, 2018
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A novel method for source-specific hemoglobin adducts of nitro-polycyclic aromatic hydrocarbons

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Abstract

Polycyclic aromatic hydrocarbons (PAH) are ubiquitous air pollutants associated with negative impacts on growth, development and behavior in children. Source-specific biological markers of PAH exposure are needed for targeting interventions to protect children. Nitro-derivatives of PAH can act as markers of exposure to diesel exhaust, gasoline exhaust, or general combustion sources. Using a novel HPLC-APCI-MS/MS detection method, we examined four hemoglobin (Hb) adducts of nitro-PAH metabolites and the Hb adduct of a benzo[a]pyrene (BaP) metabolite in 22 umbilical cord blood samples. The samples were collected from a birth cohort with comprehensive data on prenatal PAH exposure, including prenatal personal air monitoring and DNA adducts in maternal and umbilical cord blood. Using non-parametric analyses, heat maps, and principal component analysis (PCA), we analyzed the relationship between the five Hb adducts and previous PAH measurements, with each measurement representing a different duration of exposure. We found that Hb adducts derived from several diesel-related nitro-PAHs (2-nitrofluorene and 1-nitropyrene) were significantly correlated (r = 0.77, p ≤ 0.0001) and grouped together in PCA. Nitro-PAH derived Hb adducts were largely unrelated to previously collected measures of exposure to a number of PAH parent compounds. These measures need to be validated in a larger sample.

Graphical abstract: A novel method for source-specific hemoglobin adducts of nitro-polycyclic aromatic hydrocarbons

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Supplementary files

Article information


Submitted
31 Oct 2017
Accepted
18 Feb 2018
First published
21 Mar 2018

Environ. Sci.: Processes Impacts, 2018,20, 780-789
Article type
Paper

A novel method for source-specific hemoglobin adducts of nitro-polycyclic aromatic hydrocarbons

K. Wheelock, J. (. Zhang, R. McConnell, D. Tang, H. E. Volk, Y. Wang, J. B. Herbstman, S. Wang, D. H. Phillips, D. Camann, J. Gong and F. Perera, Environ. Sci.: Processes Impacts, 2018, 20, 780
DOI: 10.1039/C7EM00522A

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