Jump to main content
Jump to site search


Direct visible light activation of a surface cysteine-engineered [NiFe]-hydrogenase by silver nanoclusters

Author affiliations

Abstract

Genetically engineering a cysteine (thiolate) close to the distal [4Fe–4S] cluster of a [NiFe]-hydrogenase creates a highly specific target for attachment of Ag nanoclusters templated in polymethyl acrylate, the resulting ‘hard-wired’ enzyme catalysing rapid hydrogen evolution by visible light. The rate is further enhanced by binding to metal oxide nanoparticles – results of investigations focusing on P-25 TiO2 and including anatase TiO2, rutile TiO2, ZnO, SrTiO3 and ZrO2 leading to the proposal that these act as active or structural scaffolds to promote intra-assembly electron transfer.

Graphical abstract: Direct visible light activation of a surface cysteine-engineered [NiFe]-hydrogenase by silver nanoclusters

Back to tab navigation

Supplementary files

Publication details

The article was received on 13 Aug 2018, accepted on 14 Sep 2018 and first published on 19 Oct 2018


Article type: Communication
DOI: 10.1039/C8EE02361A
Citation: Energy Environ. Sci., 2018, Advance Article
  • Open access: Creative Commons BY license
  •   Request permissions

    Direct visible light activation of a surface cysteine-engineered [NiFe]-hydrogenase by silver nanoclusters

    L. Zhang, S. E. Beaton, S. B. Carr and F. A. Armstrong, Energy Environ. Sci., 2018, Advance Article , DOI: 10.1039/C8EE02361A

    This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. Material from this article can be used in other publications provided that the correct acknowledgement is given with the reproduced material.

    Reproduced material should be attributed as follows:

    • For reproduction of material from NJC:
      [Original citation] - Published by The Royal Society of Chemistry (RSC) on behalf of the Centre National de la Recherche Scientifique (CNRS) and the RSC.
    • For reproduction of material from PCCP:
      [Original citation] - Published by the PCCP Owner Societies.
    • For reproduction of material from PPS:
      [Original citation] - Published by The Royal Society of Chemistry (RSC) on behalf of the European Society for Photobiology, the European Photochemistry Association, and RSC.
    • For reproduction of material from all other RSC journals:
      [Original citation] - Published by The Royal Society of Chemistry.

    Information about reproducing material from RSC articles with different licences is available on our Permission Requests page.

Search articles by author

Spotlight

Advertisements