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Issue 11, 2018
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Generation of a functional, semisynthetic [FeFe]-hydrogenase in a photosynthetic microorganism

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Abstract

[FeFe]-Hydrogenases are hydrogen producing metalloenzymes with excellent catalytic capacities, highly relevant in the context of a future hydrogen economy. Here we demonstrate the synthetic activation of a heterologously expressed [FeFe]-hydrogenase in living cells of Synechocystis PCC 6803, a photoautotrophic microbial chassis with high potential for biotechnological energy applications. H2-Evolution assays clearly show that the non-native, semi-synthetic enzyme links to the native metabolism in living cells.

Graphical abstract: Generation of a functional, semisynthetic [FeFe]-hydrogenase in a photosynthetic microorganism

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Supplementary files

Article information


Submitted
06 Jul 2018
Accepted
21 Sep 2018
First published
25 Sep 2018

This article is Open Access

Energy Environ. Sci., 2018,11, 3163-3167
Article type
Communication

Generation of a functional, semisynthetic [FeFe]-hydrogenase in a photosynthetic microorganism

A. Wegelius, N. Khanna, C. Esmieu, G. D. Barone, F. Pinto, P. Tamagnini, G. Berggren and P. Lindblad, Energy Environ. Sci., 2018, 11, 3163
DOI: 10.1039/C8EE01975D

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