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pH dependent binding in de novo hetero bimetallic coiled coils

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Abstract

Herein the first example of a bimetallic coiled coil featuring a lanthanide binding site is reported, opening opportunities to exploit the attractive NMR and photophysical properties of the lanthanides in multi metallo protein design. In our efforts to fully characterise the system we identified for the first time that lanthanide binding to such sites is pH dependent, with optimal binding at neutral pH, and that the double AsnAsp site is more versatile in this regard than the single Asp site. Our second site featured the structural HgCys3 site, the chemistry of which was essentially unaltered by the presence of the lanthanide site. In fact, both metal binding sites within the hetero bimetallic coiled coil displayed the same properties as their mononuclear single binding site controls, and operated independently of each other. Finally, pH can be used as an external trigger to control the binding of Hg(II) and Tb(III) to the two distinct sites within this coiled coil, and offers the opportunity to “activate” metal binding sites within complex multi metallo and multi-functional designs.

Graphical abstract: pH dependent binding in de novo hetero bimetallic coiled coils

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Publication details

The article was received on 20 Apr 2018, accepted on 05 Jul 2018 and first published on 05 Jul 2018


Article type: Paper
DOI: 10.1039/C8DT01568F
Citation: Dalton Trans., 2018, Advance Article
  • Open access: Creative Commons BY license
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    pH dependent binding in de novo hetero bimetallic coiled coils

    P. Teare, C. F. Smith, S. J. Adams, S. Anbu, B. Ciani, L. J. C. Jeuken and A. F. A. Peacock, Dalton Trans., 2018, Advance Article , DOI: 10.1039/C8DT01568F

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