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The role of hydrophobic, aromatic and electrostatic interactions between amino acid residues and a titanium dioxide surface

Abstract

Understanding the nature of interactions between inorganic surfaces and biomolecules such as, amino acids and peptides, can enhance the development of new materials. Here, we present single force spectroscopy (SMFS) measurements of the interactions between atomic force microscopy (AFM) probe, modified with various amino acids and a titanium dioxide surface. Specifically, we study the affinity of amino acids toward titanium dioxide surface bearing hydrophobic (Leu), aromatic (Phe) and hydrophilic (Orn) residues. We find that aromatic interactions dominate over aliphatic in their affinity to titanium dioxide surface. In addition, we show that by combining aromatic and hydrophilic moieties in a single amino acid (NH2-Phe), the adhesion of the latter to the surface increases. Furthermore, the affinity of positively charged amino acids to titanium dioxide surface is higher than of uncharged, and can be increased more, with elevating the pH of the buffer above the pKa of the basic residues. The kinetic and thermodynamic parameters imply that the dynamics of the surface-amino acids interface are mostly govern by hydrophobic interactions.

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Publication details

The article was received on 12 Sep 2018, accepted on 05 Nov 2018 and first published on 06 Nov 2018


Article type: Paper
DOI: 10.1039/C8CP05775C
Citation: Phys. Chem. Chem. Phys., 2018, Accepted Manuscript
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    The role of hydrophobic, aromatic and electrostatic interactions between amino acid residues and a titanium dioxide surface

    A. Leader, D. Mandler and M. Reches, Phys. Chem. Chem. Phys., 2018, Accepted Manuscript , DOI: 10.1039/C8CP05775C

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