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The impact of lipid oxidation on functioning of a lung surfactant model

Abstract

Apart from being responsible for sufficient pulmonary compliance and preventing alveolar collapse, lung surfactant (LS) also forms the first barrier for uptake of inhaled pathogens. As such it is susceptible to damage caused by various deleterious compounds present in air, e.g. oxidants capable of oxidizing unsaturated LS lipids. This study examines the consequences of oxidizing 20% of unsaturated lipids in an LS model: a mixed 1:1 DPPC:POPC monolayer. POxnoPC (1-palmitoyl-2-(9-oxo-nonanoyl)-sn-glycero-3-phosphocholine) is considered as the main oxidation product. Experimental surface pressure-area isotherms and polarization-modulation infrared reflection-absorption spectroscopy are employed to probe changes in the macroscopic properties of unsaturated lipid monolayer induced by oxidation. Microscopic details of the oxidation influence on monolayer’s phase behavior are elucidated by molecular dynamics simulations at varying lipid packing. We demonstrate that unsaturated lipids oxidation shifts the isotherm towards larger areas and surface pressures. It also advances monolayer collapse. This is caused by reversal of oxidized sn-2 chains of POxnoPC towards the subphase, driven by electrostatic interactions between the aldehyde, glycerin, and water. Increased lipid bulkiness, hindered transition to LC phase, and transfer of oxidized lipids to the subphase have been identified as the most troublesome consequences of this process. They result in reduction of monolayer stability and its capability to withstand high surface pressures. This may lead to uncontrolled and irreversible loss of lipids from the surface.

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Publication details

The article was received on 16 Jul 2018, accepted on 11 Sep 2018 and first published on 12 Sep 2018


Article type: Paper
DOI: 10.1039/C8CP04496A
Citation: Phys. Chem. Chem. Phys., 2018, Accepted Manuscript
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    The impact of lipid oxidation on functioning of a lung surfactant model

    B. Korchowiec, A. Stachowicz, L. Cwiklik, J. Korchowiec and E. Rogalska, Phys. Chem. Chem. Phys., 2018, Accepted Manuscript , DOI: 10.1039/C8CP04496A

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