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Issue 44, 2018
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Structural characterization of nucleotide 5′-triphosphates by infrared ion spectroscopy and theoretical studies

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Abstract

The molecular family of nucleotide triphosphates (NTPs), with adenosine 5′-triphosphate (ATP) as its best-known member, is of high biochemical importance as their phosphodiester bonds form Nature's main means to store and transport energy. Here, gas-phase IR spectroscopic studies and supporting theoretical studies have been performed on adenosine 5′-triphosphate, cytosine 5′-triphosphate and guanosine 5′-triphosphate to elucidate the intrinsic structural properties of NTPs, focusing on the influence of the nucleobase and the extent of deprotonation. Mass spectrometric studies involving collision induced dissociation showed similar fragmentation channels for the three studied NTPs within a selected charge state. The doubly charged anions exhibit fragmentation similar to the energy-releasing hydrolysis reaction in nature, while the singly charged anions show different dominant fragmentation channels, suggesting that the charge state plays a significant role in the favorability of the hydrolysis reaction. A combination of infrared ion spectroscopy and quantum-chemical computations indicates that the singly charged anions of all NTPs are preferentially deprotonated at their β-phosphates, while the doubly-charged anions are dominantly αβ-deprotonated. The assigned three-dimensional structure differs for ATP and CTP on the one hand and GTP on the other, in the sense that ATP and CTP show no interaction between nucleobase and phosphate tail, while in GTP they are hydrogen bonded. This can be rationalized by considering the structure and geometry of the NTPs where the final three dimensional structure depends on a subtle balance between hydrogen bond strength, flexibility and steric hindrance.

Graphical abstract: Structural characterization of nucleotide 5′-triphosphates by infrared ion spectroscopy and theoretical studies

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Publication details

The article was received on 24 May 2018, accepted on 24 Oct 2018 and first published on 25 Oct 2018


Article type: Paper
DOI: 10.1039/C8CP03314E
Citation: Phys. Chem. Chem. Phys., 2018,20, 28319-28330
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    Structural characterization of nucleotide 5′-triphosphates by infrared ion spectroscopy and theoretical studies

    R. E. van Outersterp, J. Martens, G. Berden, J. D. Steill, J. Oomens and A. M. Rijs, Phys. Chem. Chem. Phys., 2018, 20, 28319
    DOI: 10.1039/C8CP03314E

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