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A structural model of the hierarchical assembly of an amyloid nanosheet by an infrared probe technique

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Abstract

Rational design of amyloid-based materials requires structural insight into such materials. Here, we explore the use of a side-chain-based infrared (IR) probe technique combined with atomic force microscopy, Raman spectroscopy, UV-Vis spectroscopy, and thermogravimetric analysis coupled with mass spectrometry to elucidate the structural details of an amyloid nanosheet formed by an Aβ(16–22) variant, KLVXFAK, where X is p-cyanophenylalanine with its side-chain cyano group being an IR probe. Through the structural constraints obtained with the combined tools, we are able to propose a novel structural model for the amyloid nanosheet. The nanosheet can be viewed as a stack of class 7-type steric-zipper-like amyloid structures with a unique sheared intersheet arrangement: the β-sheets are stacked along a zippering axis with each individual β-sheet sheared relative to its adjacent β-sheets by two residues through the cyano–lysine intersheet hydrogen bond. With such a configuration, the side view of the nanosheet is similar to that of a stack of tilted-aligned roof tiles with each individual β-sheet being each tile. In addition, this work provides a nice example of how to utilize the side-chain-based IR probe technique combined with other supplemental tools to build a hierarchical structural model for a complex amyloid assembly.

Graphical abstract: A structural model of the hierarchical assembly of an amyloid nanosheet by an infrared probe technique

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Publication details

The article was received on 11 May 2018, accepted on 11 Aug 2018 and first published on 14 Aug 2018


Article type: Paper
DOI: 10.1039/C8CP03003K
Citation: Phys. Chem. Chem. Phys., 2018, Advance Article
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    A structural model of the hierarchical assembly of an amyloid nanosheet by an infrared probe technique

    B. Jia, Y. Sun, L. Yang, Y. Yu, H. Fan and G. Ma, Phys. Chem. Chem. Phys., 2018, Advance Article , DOI: 10.1039/C8CP03003K

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