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SecScan: a general approach for mapping disulfide bonds in synthetic and recombinant peptides and proteins

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Abstract

Selenocysteine scanning (SecScan) is a novel technique to map disulfide networks in proteins independent of structure-based distance information and mass spectrometry. SecScan applies systematic substitution of single Cys by Sec in combination with NMR spectroscopy for reliable and unambiguous determination of disulfide bond networks.

Graphical abstract: SecScan: a general approach for mapping disulfide bonds in synthetic and recombinant peptides and proteins

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Publication details

The article was received on 03 Nov 2018, accepted on 28 Nov 2018 and first published on 28 Nov 2018


Article type: Communication
DOI: 10.1039/C8CC08777F
Citation: Chem. Commun., 2019, Advance Article
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    SecScan: a general approach for mapping disulfide bonds in synthetic and recombinant peptides and proteins

    S. S. Denisov, J. H. Ippel, B. J. Mans, I. Dijkgraaf and T. M. Hackeng, Chem. Commun., 2019, Advance Article , DOI: 10.1039/C8CC08777F

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