Issue 97, 2018
From the journal:

Chemical Communications

A dual signal amplification-assisted DNAzyme biosensor for ultrasensitive detection of Argonaute 2 activity†

Dandan Zhang,‡a   Fei Ma,‡a   Junhong Leng‡b  and  Chun-yang Zhang ORCID logo *a  

* Corresponding authors

a College of Chemistry, Chemical Engineering and Materials Science, Collaborative Innovation Center of Functionalized Probes for Chemical Imaging in Universities of Shandong, Key Laboratory of Molecular and Nano Probes, Ministry of Education, Shandong Provincial Key Laboratory of Clean Production of Fine Chemicals, Shandong Normal University, Jinan 250014, China
E-mail: cyzhang@sdnu.edu.cn
Fax: +86 0531-82615258
Tel: +86 0531-86186033

b Jinan Maternity and Child Care Hospital, Jinan 250000, China

Abstract

We develop a new DNAzyme biosensor for Argonaute 2 (Ago2) assay based on target-initiated rolling circle amplification in combination with an 8-17 DNAzyme-mediated dual signal amplification strategy. This biosensor can achieve ultrahigh sensitivity with a detection limit of 0.35 pM, and it can be further used for the screening of Ago2 inhibitors and the accurate measurement of endogenous Ago2 from HeLa cell extracts.

Graphical abstract: A dual signal amplification-assisted DNAzyme biosensor for ultrasensitive detection of Argonaute 2 activity

About
Cited by
Related
Download options Please wait...

Supplementary files

Article information

DOI
https://doi.org/10.1039/C8CC08553F
Article type
Communication
Submitted
25 Oct 2018
Accepted
06 Nov 2018
First published
13 Nov 2018

Chem. Commun., 2018,54, 13678-13681

Permissions

Request permissions

A dual signal amplification-assisted DNAzyme biosensor for ultrasensitive detection of Argonaute 2 activity

D. Zhang, F. Ma, J. Leng and C. Zhang, Chem. Commun., 2018, 54, 13678 DOI: 10.1039/C8CC08553F

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements