Quantitative analysis of cell-free synthesized membrane proteins at the stabilized droplet interface bilayer

Maie A. Elfaramawy; Satoshi Fujii; Atsuko Uyeda; Toshihisa Osaki; Shoji Takeuchi; Yasuhiko Kato; Hajime Watanabe; Tomoaki Matsuura

doi:10.1039/C8CC06804F

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Quantitative analysis of cell-free synthesized membrane proteins at the stabilized droplet interface bilayer†

Maie A. Elfaramawy,^a Satoshi Fujii,^b Atsuko Uyeda,^a Toshihisa Osaki,

^bc Shoji Takeuchi,

^bc Yasuhiko Kato,^a Hajime Watanabe^a and Tomoaki Matsuura

*^a

Author affiliations

* Corresponding authors

^a Department of Biotechnology, Division of Advance Science and Biotechnology, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, 565-0871 Osaka, Japan
E-mail: matsuura_tomoaki@bio.eng.osaka-u.ac.jp

^b Artificial Cell Membrane Systems Group, Kanagawa Institute of Industrial Science and Technology, 3-2-1 Sakado, Takatsu, 213-0012 Kawasaki, Japan

^c Institute of Industrial Science, The University of Tokyo, 4-6-1 Komaba, Meguro, 153-8505 Tokyo, Japan

Abstract

We report the functional synthesis and quantification of membrane proteins—α-hemolysin from Staphylococcus aureus and the multidrug transporter EmrE from Escherichia coli—at the stabilized droplet interface bilayer using an in vitro transcription–translation system. The system developed here can expand the list of integral membrane proteins applicable for quantitative functional analysis.

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Article information

DOI: https://doi.org/10.1039/C8CC06804F
Article type: Communication
Submitted: 22 Aug 2018
Accepted: 05 Oct 2018
First published: 08 Oct 2018

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Chem. Commun., 2018,54, 12226-12229

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Quantitative analysis of cell-free synthesized membrane proteins at the stabilized droplet interface bilayer

M. A. Elfaramawy, S. Fujii, A. Uyeda, T. Osaki, S. Takeuchi, Y. Kato, H. Watanabe and T. Matsuura, Chem. Commun., 2018, 54, 12226 DOI: 10.1039/C8CC06804F

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Chemical Communications

Quantitative analysis of cell-free synthesized membrane proteins at the stabilized droplet interface bilayer†

Abstract

Supplementary files

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Quantitative analysis of cell-free synthesized membrane proteins at the stabilized droplet interface bilayer

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