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Issue 81, 2018
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Directed sortase A evolution for efficient site-specific bioconjugations in organic co-solvents

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Abstract

Directed sortase A evolution yielded the variants R159G and D165Q/D186G/K196V with increased resistance (2.2-fold) and catalytic efficiency (6.3-fold) in 45% (v/v) dimethylsulfoxide. Interestingly, D165Q/D186G/K196V also showed an up to 4.7-fold increased activity for the conjugation of hydrophobic peptides/amines in co-solvents. MD simulations revealed that conformational mobilities are important for the gained resistance.

Graphical abstract: Directed sortase A evolution for efficient site-specific bioconjugations in organic co-solvents

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Article information


Submitted
24 Jul 2018
Accepted
19 Sep 2018
First published
20 Sep 2018

Chem. Commun., 2018,54, 11467-11470
Article type
Communication

Directed sortase A evolution for efficient site-specific bioconjugations in organic co-solvents

Z. Zou, H. Alibiglou, D. M. Mate, M. D. Davari, F. Jakob and U. Schwaneberg, Chem. Commun., 2018, 54, 11467
DOI: 10.1039/C8CC06017G

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