Jump to main content
Jump to site search

Issue 71, 2018
Previous Article Next Article

ESIPT-based ratiometric fluorescence probe for the intracellular imaging of peroxynitrite

Author affiliations

Abstract

In this work, we set out to develop an endoplasmic reticulum (ER) directed ESIPT-based ONOO ratiometric fluorescent probe (ABAH-LW). ABAH-LW was synthesized in four steps and found to have a high sensitivity and selectivity towards the detection of ONOO. ABAH-LW was able to detect low concentrations of ONOO (limit of detection = 21.4 nM) within seconds producing a ratiometric change in fluorescence intensity. ABAH-LW further demonstrated the ability to ratiometrically image endogenous and exogenous ONOO in HeLa cells. Moreover, co-localization experiments were carried out using commercially available ER-Tracker Red, Lyso-Tracker Red and Mito-Tracker-Red, which were co-stained with ABAH-LW in HeLa cells. For ER-Tracker Red, Pearson's correlation co-efficient of 0.93 was determined and 3D surface plot analysis illustrated a large overlap between ABAH-LW and ER-Tracker Red using both red and blue channels. In addition some co-localisation with Mito-Tracker Red and ABAH-LW was observed (0.73).

Graphical abstract: ESIPT-based ratiometric fluorescence probe for the intracellular imaging of peroxynitrite

Back to tab navigation

Supplementary files

Publication details

The article was received on 20 Jun 2018, accepted on 03 Aug 2018 and first published on 07 Aug 2018


Article type: Communication
DOI: 10.1039/C8CC04919J
Citation: Chem. Commun., 2018,54, 9953-9956
  • Open access: Creative Commons BY license
  •   Request permissions

    ESIPT-based ratiometric fluorescence probe for the intracellular imaging of peroxynitrite

    L. Wu, Y. Wang, M. Weber, L. Liu, A. C. Sedgwick, S. D. Bull, C. Huang and T. D. James, Chem. Commun., 2018, 54, 9953
    DOI: 10.1039/C8CC04919J

    This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. Material from this article can be used in other publications provided that the correct acknowledgement is given with the reproduced material.

    Reproduced material should be attributed as follows:

    • For reproduction of material from NJC:
      [Original citation] - Published by The Royal Society of Chemistry (RSC) on behalf of the Centre National de la Recherche Scientifique (CNRS) and the RSC.
    • For reproduction of material from PCCP:
      [Original citation] - Published by the PCCP Owner Societies.
    • For reproduction of material from PPS:
      [Original citation] - Published by The Royal Society of Chemistry (RSC) on behalf of the European Society for Photobiology, the European Photochemistry Association, and RSC.
    • For reproduction of material from all other RSC journals:
      [Original citation] - Published by The Royal Society of Chemistry.

    Information about reproducing material from RSC articles with different licences is available on our Permission Requests page.

Search articles by author

Spotlight

Advertisements