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Issue 79, 2018
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Enantio- and regioselective ene-reductions using F420H2-dependent enzymes

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Abstract

In the past decade it has become clear that many microbes harbor enzymes that employ an unusual flavin cofactor, the F420 deazaflavin cofactor. Herein we show that F420-dependent reductases (FDRs) can successfully perform enantio-, regio- and chemoselective ene-reductions. For the first time, we have demonstrated that F420H2-driven reductases can be used as biocatalysts for the reduction of α,β-unsaturated ketones and aldehydes with good conversions (>99%) and excellent regioselectivities and enantiomeric excesses (>99% ee). Noteworthily, FDRs typically display an opposite enantioselectivity when compared to the well established FMN-dependent Old Yellow Enzymes (OYEs).

Graphical abstract: Enantio- and regioselective ene-reductions using F420H2-dependent enzymes

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Publication details

The article was received on 04 Jun 2018, accepted on 12 Sep 2018 and first published on 12 Sep 2018


Article type: Communication
DOI: 10.1039/C8CC04449J
Citation: Chem. Commun., 2018,54, 11208-11211
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    Enantio- and regioselective ene-reductions using F420H2-dependent enzymes

    S. Mathew, M. Trajkovic, H. Kumar, Q. Nguyen and M. W. Fraaije, Chem. Commun., 2018, 54, 11208
    DOI: 10.1039/C8CC04449J

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