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The concept of a relay FRET event is established in a designed trichromophoric pentapeptide containing an o-,m-aromatic amino acid scaffold in the backbone as a novel β-turn mimetic β-sheet folding nucleator. This system would find application in studying fundamental processes involving interbiomolecular interactions in chemical biology.

Graphical abstract: A relay FRET event in a designed trichromophoric pentapeptide containing an o-,m-aromatic-amino acid scaffold

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