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Sensitive and label-free discrimination of 5-hydroxymethylcytosine and 5-methylcytosine in DNA by ligation-mediated rolling circle amplification

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Abstract

We develop a label-free fluorescence method for sensitive discrimination of 5-hydroxymethylcytosine (5hmC) and 5-methylcytosine (5mC) based on the specific oxidation of 5hmC to 5-formylcytosine (5fC) and the subsequent conversion to uracil (U) in combination with ligation-mediated rolling circle amplification (RCA). This method exhibits high sensitivity with a detection limit of as low as 34.8 fM. It can discriminate as low as the 0.01% 5hmC level from the mixture, and exhibits good performance in serum sample analysis as well.

Graphical abstract: Sensitive and label-free discrimination of 5-hydroxymethylcytosine and 5-methylcytosine in DNA by ligation-mediated rolling circle amplification

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Publication details

The article was received on 17 May 2018, accepted on 07 Jul 2018 and first published on 10 Jul 2018


Article type: Communication
DOI: 10.1039/C8CC03938K
Citation: Chem. Commun., 2018, Advance Article
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    Sensitive and label-free discrimination of 5-hydroxymethylcytosine and 5-methylcytosine in DNA by ligation-mediated rolling circle amplification

    Z. Wang, M. Wang, Y. Zhang and C. Zhang, Chem. Commun., 2018, Advance Article , DOI: 10.1039/C8CC03938K

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