Issue 61, 2018

ESIPT-based fluorescence probe for the rapid detection of hypochlorite (HOCl/ClO)


ESIPT-based fluorescence probes are emerging as an attractive tool for the detection of biologically relevant analytes owing to their unique photophysical properties. In this work, we have developed an ESIPT-based fluorescence probe (TCBT-OMe) for the detection of HClO/ClO through the attachment of a bioorthogonal dimethylthiocarbamate linker. TCBT-OMe was shown to rapidly detect HClO/ClO (<10 s) at biologically relevant concentrations (LoD = 0.16 nM) and have an excellent selectivity towards others ROS/RNS and amino acids. Therefore, TCBT-OMe was tested in live cells and was successfully shown to be able to detect endogenous and exogenous HClO/ClO in HeLa cells. Additionally, TCBT-OMe acts as a dual input logic gate for Hg2+ and H2O2. Interestingly, Hg2+ alone gradually causes a fluorescence response but requires >30 min to produce a fluorescence response. Test strips containing TCBT-OMe were prepared and were demonstrated as an effective way to detect HClO/ClO in water. Furthermore, TCBT-OMe was shown to detect exogenously added HClO/ClO in three different water samples with little interference thus demonstrating the effectiveness as a method for the detection of HClO/ClO in drinking water samples.

Graphical abstract: ESIPT-based fluorescence probe for the rapid detection of hypochlorite (HOCl/ClO−)

Supplementary files

Article information

Article type
08 May 2018
28 Jun 2018
First published
04 Jul 2018
This article is Open Access
Creative Commons BY license

Chem. Commun., 2018,54, 8522-8525

ESIPT-based fluorescence probe for the rapid detection of hypochlorite (HOCl/ClO)

L. Wu, Q. Yang, L. Liu, A. C. Sedgwick, A. J. Cresswell, S. D. Bull, C. Huang and T. D. James, Chem. Commun., 2018, 54, 8522 DOI: 10.1039/C8CC03717E

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