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Issue 66, 2018
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A bacterial biosensor encoding a genetically modified LuxR receptor exhibits improved detection of Pseudomonas aeruginosa's biomarker molecule 2-aminoacetophenone

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Abstract

2-Aminoacetophneone (2-AA) is a volatile molecule produced in high amounts by the opportunistic pathogen Pseudomonas aeruginosa. We have previously shown that 2-AA activates the quorum sensing (QS) LuxR receptor of Aliivibrio fischeri. In the present study we were able to improve LuxR's affinity and detection limit for 2-AA by genetic modification of three amino acids within the binding pocket of the receptor. Expression of the modified LuxR receptor in a luminescent bacterial biosensor provided an efficient detection assay of 2-AA in clinical P. aeruginosa strains isolated from blood and lung infections, as well as in phlegm samples obtained from subjects suffering from lung infections.

Graphical abstract: A bacterial biosensor encoding a genetically modified LuxR receptor exhibits improved detection of Pseudomonas aeruginosa's biomarker molecule 2-aminoacetophenone

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Supplementary files

Article information


Submitted
02 May 2018
Accepted
24 Jul 2018
First published
26 Jul 2018

Chem. Commun., 2018,54, 9218-9221
Article type
Communication

A bacterial biosensor encoding a genetically modified LuxR receptor exhibits improved detection of Pseudomonas aeruginosa's biomarker molecule 2-aminoacetophenone

I. Kviatkovski, T. Yarnitzky, S. Shushan, O. Schwartz-Harari, R. Nir-Paz and Y. Helman, Chem. Commun., 2018, 54, 9218
DOI: 10.1039/C8CC03540G

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