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Issue 38, 2018
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Long-wavelength TCF-based fluorescence probes for the detection and intracellular imaging of biological thiols

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Abstract

Two ‘turn on’ TCF-based fluorescence probes were developed for the detection of biological thiols (TCF-GSH and TCFCl-GSH). TCF-GSH was shown to have a high sensitivity towards glutathione (GSH) with a 0.28 μM limit of detection. Unfortunately, at higher GSH concentrations the fluorescence intensity of TCF-GSH decreased and toxicity was observed for TCF-GSH in live cells. However, TCFCl-GSH was shown to be able to detect GSH at biologically relevant concentrations with a 0.45 μM limit of detection. No toxicity was found for TCFCl-GSH and a clear ‘turn on’ with good photostability was observed for the exogenous addition of GSH, Cys and HCys. Furthermore, TCFCl-GSH was used to evaluate the effects of drug treatment on the levels of GSH in live cells.

Graphical abstract: Long-wavelength TCF-based fluorescence probes for the detection and intracellular imaging of biological thiols

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Supplementary files

Article information


Submitted
28 Feb 2018
Accepted
16 Apr 2018
First published
17 Apr 2018

This article is Open Access

Chem. Commun., 2018,54, 4786-4789
Article type
Communication

Long-wavelength TCF-based fluorescence probes for the detection and intracellular imaging of biological thiols

A. C. Sedgwick, J. E. Gardiner, G. Kim, M. Yevglevskis, M. D. Lloyd, A. T. A. Jenkins, S. D. Bull, J. Yoon and T. D. James, Chem. Commun., 2018, 54, 4786
DOI: 10.1039/C8CC01661E

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