Orthogonal supramolecular protein assembly on patterned bifunctional surfaces

Abstract

We report successful and selective dual protein assembly on patterned bifunctional βCD-Ni(II)NTA surfaces, using red fluorescent protein variants with hexahistidine-tags and teal fluorescent protein variants conjugated with a peptide containing three adamantyl groups. We show that dual protein patterns can only be assembled, when opposing supramolecular interactions have been optimized and nonspecific interactions have been sufficiently suppressed.