Counting DNA molecules with visual segment-based readouts in minutes

Rui Wang; Fang Zhang; Cheng Qian; Cui Wu; Zunzhong Ye; Liu Wang; Wenjuan Qian; Jianfeng Ping; Jian Wu; Yibin Ying

doi:10.1039/C7CC09515E

Counting DNA molecules with visual segment-based readouts in minutes†

Rui Wang,

‡^a Fang Zhang,‡^b Cheng Qian,^a Cui Wu,^a Zunzhong Ye,^a Liu Wang,^a Wenjuan Qian,^a Jianfeng Ping,^a Jian Wu*^a and Yibin Ying^ac

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* Corresponding authors

^a College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou, China
E-mail: wujian69@zju.edu.cn
Fax: +86-571-88982180
Tel: +86-571-88982180

^b College of Biological Science and Engineering, Fuzhou University, Fuzhou, China

^c Faculty of Agricultural and Food Science, Zhejiang A & F University, Hangzhou, China

Abstract

An ultrafast and extremely simple approach was proposed to count the number of DNA molecules without any microfluidic-based device. By directly counting the number of amplicon clusters in a capillary, the absolute amount of DNA molecules could be easily determined.

Chemical Communications

Counting DNA molecules with visual segment-based readouts in minutes†

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Counting DNA molecules with visual segment-based readouts in minutes

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