DNAzyme based visual detection of DNA methylation

Mei Li; Lei Zhang; Gangyi Chen; Jing Zhou; Yi Yuan; Jiawei Zou; Mingming Yuan; Rong Chen; Feng Du; Xin Cui; Xin Huang; Juan Dong; Zhuo Tang

doi:10.1039/C7CC08427G

DNAzyme based visual detection of DNA methylation†

Mei Li,^a Lei Zhang,^b Gangyi Chen,^a Jing Zhou,^b Yi Yuan,^a Jiawei Zou,^a Mingming Yuan,^b Rong Chen,^a Feng Du,^a Xin Cui,^a Xin Huang,^a Juan Dong^a and Zhuo Tang

*^a

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* Corresponding authors

^a Natural Products Research Center, Chengdu Institute of Biology, University of Chinese Academy of Sciences, Chengdu, Sichuan, P. R. China
E-mail: tangzhuo@cib.ac.cn

^b Sichuan Academy of Chinese Medicine Sciences, Chengdu, Sichuan, P. R. China

Abstract

A novel colorimetric method which utilizes DNAzyme as a signal reporter was developed for DNA methylation detection. As low as 1/10⁶ of methylated DNA could be successfully detected from unmethylated DNA. The discrimination ability is at least two orders of magnitude better than that of the methylation specific PCR.

Chemical Communications

DNAzyme based visual detection of DNA methylation†

Abstract

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DNAzyme based visual detection of DNA methylation

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