Issue 15, 2018
From the journal:

Chemical Communications

Enzymatically cleavable traceless biotin tags for protein PEGylation and purification†

Adam M. Wawro, ORCID logo ab   Yusuke Aoki,a   Takahiro Muraoka, ORCID logo *ac   Kouhei Tsumotobde  and  Kazushi Kinbara ORCID logo *a  

* Corresponding authors

a School of Life Science and Technology, Tokyo Institute of Technology, Yokohama 226-8501, Japan
E-mail: tmuraoka@bio.titech.ac.jp, kkinbara@bio.titech.ac.jp

b Department of Bioengineering, School of Engineering, The University of Tokyo, Tokyo 108-8656, Japan

c PRESTO, Japan Science and Technology Agency, Saitama 332-0012, Japan

d Department of Chemistry and Biotechnology, School of Engineering, The University of Tokyo, Tokyo 108-8656, Japan

e Institute of Medical Science, The University of Tokyo, Tokyo 108-8639, Japan

Abstract

Here we report an example of a protein–PEG conjugate with a biotin tag cleavable by lipase-catalyzed hydrolysis. Very mild cleavage conditions, heterogeneous, easily separable catalysts, and traceless design make this method attractive for the preparation and purification of PEGylated proteins.

Graphical abstract: Enzymatically cleavable traceless biotin tags for protein PEGylation and purification

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Article information

DOI
https://doi.org/10.1039/C7CC05814D
Article type
Communication
Submitted
26 Jul 2017
Accepted
05 Jan 2018
First published
08 Jan 2018

Chem. Commun., 2018,54, 1913-1916

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Enzymatically cleavable traceless biotin tags for protein PEGylation and purification

A. M. Wawro, Y. Aoki, T. Muraoka, K. Tsumoto and K. Kinbara, Chem. Commun., 2018, 54, 1913 DOI: 10.1039/C7CC05814D

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