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Treatment of Dry eye by Intracanalicular injection of a Thermosensitive Chitosan-based Hydrogel: Evaluation of Biosafety and Availability

Abstract

Chitosan has been increasingly considered for the design of implant materials in the field of translational medicine. However, implant properties addressing the complexity of the desired tissue still need to be developed. The focus of this study lies in the evaluation of a thermosensitive Chitosan-based Hydrogel for intracanalicular injection. Hydroxybutyl chitosan (HBC) solution was prepared, and cytocompatibility was investigated by the CCK-8 assay using primary human corneal epithelial cells (HCEpiCs). Minimal cytotoxicity was seen in cultures with HBC-extracting solution at concentration of 0.2g/ml for up to 72 h incubation. Biocompatibility and effectiveness, based on both rabbit model and human pilot study, were evaluated anatomically and functionally. The flow flux significantly decreased after HBC injection, with 76.9% of the flow flux occurring 10 min after HBC injection. Tear secretion significantly improved on rabbit model. The density of PAS-positive cells gradually increased on animal model. Various clinical indicators, which include ocular surface disease index (OSDI) and tear break up time, have been improved greatly. Thermosensitivity promotes greater suitability for HBC intracanalicular injection to obstruct the lacrimal drainage system under body temperature. These results demonstrate that thermosensitive Chitosan-based Hydrogel is suitable as a liquid plug for tear flow blockage and thus represents a promising candidate for translational medicine.

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Publication details

The article was received on 30 Aug 2018, accepted on 03 Oct 2018 and first published on 05 Oct 2018


Article type: Paper
DOI: 10.1039/C8BM01047A
Citation: Biomater. Sci., 2018, Accepted Manuscript
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    Treatment of Dry eye by Intracanalicular injection of a Thermosensitive Chitosan-based Hydrogel: Evaluation of Biosafety and Availability

    T. Lin, Y. Lu, X. Zhang, L. Gong and C. Wei, Biomater. Sci., 2018, Accepted Manuscript , DOI: 10.1039/C8BM01047A

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