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Facile Synthesis, Biofilm Disruption Properties and Biocompatibility Study of Poly-cationic Peptide Functionalized Graphene-Silver Nanocomposite

Abstract

Bacterial colonization and biofilm formation is a growing challenge in biomedical fields. Nanotechnology although has emerged as an alternative strategy to combat biofilm formation, toxicity of nanomaterial is major concern. In this study we report a safe-by-design strategy for synthesis of poly-cationic peptide functionalized graphene-silver nanocomposite (designated as GAPP) and it’s enhanced anti-biofouling properties to eliminate the biofilm formation of Gram-negative bacteria. The graphene-silver (rGOAg) nanocomposite was synthesized by microwave reduction, and subsequently functionalized with antimicrobial poly-cationic peptide through covalent bonding. The results demonstrated that GAPP effectively killed the planktonic cells and biofilms of Escherichia coli, and Pseudomonas aeruginosa depending on concentration and duration of interaction. The complete eradication of preformed biofilm was achieved when treated with 10 µg/mL of GAPP for 5 h. The GAPP exerted bactericidal and biofilm inhibition activity through “contact-kill-release” mode of action, wherein electrostatic interaction of GAPP with bacterial cells induced physical disruption accompanied by ROS-mediated biochemical changes. The internalization of GAPP into the cytoplasm through the damaged membrane led to metabolic imbalance in the cells. The peptide functionalization further prevented the dissolution of Ag+ ions, and thus minimizing the cytotoxicity of GAPP to the adult zebrafish. More importantly, the poly-cationic peptide functionalization enhanced the bioavailability, biofilm inhibition and disruption activities of GAPP, while minimizing its toxicological impact. The results obtained thereby providing an effective strategy in the design of alternative antibacterial agent for fighting against biofilm of Gram-negative bacteria.

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Publication details

The article was received on 20 Aug 2018, accepted on 26 Sep 2018 and first published on 04 Oct 2018


Article type: Paper
DOI: 10.1039/C8BM01003J
Citation: Biomater. Sci., 2018, Accepted Manuscript
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    Facile Synthesis, Biofilm Disruption Properties and Biocompatibility Study of Poly-cationic Peptide Functionalized Graphene-Silver Nanocomposite

    T. Paranthaman and S. K. Das, Biomater. Sci., 2018, Accepted Manuscript , DOI: 10.1039/C8BM01003J

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