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Cationic Lipid-Assisted Nanoparticle for Delivery of mRNA Cancer Vaccine

Abstract

Message RNA-based vaccines, with their prominent advantages such as facile production, no requirement for nuclear entry and high safety without the need of integration into the host genome, have shown to be potent activators of the cytotoxic immune system. However, the wider application of mRNA-based therapeutics has been hindered because of their intrinsically high vulnerability to expressed nucleases and difficulty to enter antigen-presenting cells (APCs) directly. Here, we investigated the potential of cationic lipid-assisted nanoparticle (CLAN), a clinically translatable nucleic acid delivery system that worked as a carrier of a mRNA vaccine. We found that CLAN encapsulating mRNA encoding antigen could effectively stimulate the maturation of dendritic cells (DCs) and promote the activation and proliferation of antigen-specific T cells both in vitro and in vivo. Intravenous immunization of mice with CLAN containing mRNA encoding ovalbumin (OVA) provoked a strong OVA-specific T-cell response and slowed tumor growth in an aggressive E.G7-OVA lymphoma model. Collectively, CLAN proved to be a promising platform for mRNA vaccine delivery.

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Publication details

The article was received on 01 Aug 2018, accepted on 12 Sep 2018 and first published on 13 Sep 2018


Article type: Paper
DOI: 10.1039/C8BM00908B
Citation: Biomater. Sci., 2018, Accepted Manuscript
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    Cationic Lipid-Assisted Nanoparticle for Delivery of mRNA Cancer Vaccine

    Y. Fan, M. Li, Y. Luo, Q. Chen, L. Wang, H. Zhang, S. Shen, Z. Gu and J. Wang, Biomater. Sci., 2018, Accepted Manuscript , DOI: 10.1039/C8BM00908B

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