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Evaluation of absorbable hemostatic agents of polyelectrolyte complexes using carboxymethyl starch and chitosan oligosaccharide both in vitro and in vivo

Abstract

Absorbable hemostatic agents with high hemostatic efficacy play an important role in surgical and severely traumatic hemostasis. In the present study, by applying polyelectrolyte assembly, polyelectrolyte complexes (PECs), using carboxymethyl starch (CMS) and chitosan oligosaccharide (COS), with controllable physicochemical properties were prepared and optimized for hemorrhage control. Particle size, zeta potential, morphology and water absorption of the PECs with different CMS/COS ratio were systematically evaluated. The results of in vitro degradation in PBS suggested that CMS/COS PECs were degradable and their degradation rates, which decreased with increase of COS content, were suitable for absorbable hemostatic agents. The in vivo hemostatic efficacy of the PECs with 10 w% COS content (PEC 10), which was evaluated in a rabbit hepatic hemorrhage model, was better than CMS but decreased with increase of COS content. The plasma coagulation evaluation revealed that the PECs could significantly activate and accelerate coagulation cascade through both the intrinsic and extrinsic pathway but not directly affected the common pathway. CMS/COS PECs also showed antimicrobial activity against S. Aureus, which enhanced with the increase of COS content, but failed against E. Coli. Moreover, PEC 10 displayed excellent cytocompatibility with MC3T3-L1 and good tissue compatibility in a rabbit liver model. These findings not only suggest that CMS/COS PECs with suitable COS content was a promising absorbable hemostatic agent for internal use but also be meanful to understand the underline principles for designing PECs based hemostatic agent.

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Publication details

The article was received on 06 Jun 2018, accepted on 02 Aug 2018 and first published on 18 Sep 2018


Article type: Paper
DOI: 10.1039/C8BM00628H
Citation: Biomater. Sci., 2018, Accepted Manuscript
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    Evaluation of absorbable hemostatic agents of polyelectrolyte complexes using carboxymethyl starch and chitosan oligosaccharide both in vitro and in vivo

    X. Chen, Y. Yan, H. Li, X. Wang, S. Tang, Q. Li, J. Wei and J. Su, Biomater. Sci., 2018, Accepted Manuscript , DOI: 10.1039/C8BM00628H

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