Jump to main content
Jump to site search


Homing of ICG-loaded liposome inlaid with tumor cellular membrane to the homologous xenografts glioma eradicates the primary focus and prevents lung metastases through phototherapy

Abstract

Currently, phototherapy initiated by local irradiation of near-infrared laser has emerged as a promising strategy for cancer treatment because of low toxicity. However, how to specifically deliver a sufficient dose of photosensitizers to tumor focus is one key question for effective phototherapy. Herein, indocyanine green (ICG), a FDA-approved photosensitizer, was firstly encapsulated in inner aqueous compartment of liposome (ICG-LIP) to improve its stability. Thereafter, tumor cell membrane were isolated from native glioma cells and subsequently further inlaid in the bilayer lipid membrane of ICG-LIP to construct a cell-like liposome (ICG-MCLs). ICG was easily encapsulated into ICG-MCLs with a very high encapsulation efficiency reaching to 78.01±0.72% and its concentration in the final formulation was reaching to be 200μg/mL. ICG-MCLs displayed a spherical morphology with hydrodynamic diameter (Dh) of 115.0±0.5nm and PDI of 0.14, and Zeta potential of -11.2±0.9 mV. Moreover, ICG-MCLs exhibited a good stability of particle size and significantly improved chemical stability of ICG in pH 7.4PBS at 37 ℃. Besides, the temperature of ICG-MCLs rapidly increased to 63℃ within 10 min of radiation and maintained for a longer time. Because of cancer cell membrane associated protein, ICG-MCLs were specifically internalized by homogenous glioma C6 cells in vitro, which resulted in a strong red fluorescence of ICG in cytoplasm. Moreover, in vivo imaging showed that ICG-MCLs were effectively homed to tumor site of C6 glioma-bearing Xenograft nude mice through vein injection, which resulted in the temperature of tumor site rapidly rising up to kill tumor cells after local NIR irradiation. After treatment with ICG-MCLs, the primary tumor focus was completely eradicated and lung metastases were effectively inhibited. Conclusively, liposome inlaid with tumor cellular membrane may be served as an excellent nanoplatform for homologous-targeting phototherapy of ICG.

Back to tab navigation

Supplementary files

Publication details

The article was received on 31 May 2018, accepted on 05 Jul 2018 and first published on 11 Jul 2018


Article type: Paper
DOI: 10.1039/C8BM00604K
Citation: Biomater. Sci., 2018, Accepted Manuscript
  •   Request permissions

    Homing of ICG-loaded liposome inlaid with tumor cellular membrane to the homologous xenografts glioma eradicates the primary focus and prevents lung metastases through phototherapy

    H. Xu, B. Shen, M. Lin, M. Tong, Y. Zheng, X. Jiang, W. Yang, J. Yuan, Q. Yao and Y. Zhao, Biomater. Sci., 2018, Accepted Manuscript , DOI: 10.1039/C8BM00604K

Search articles by author

Spotlight

Advertisements