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Issue 8, 2018
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Antibiotic functionalised polymers reduce bacterial biofilm and bioburden in a simulated infection of the cornea

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Abstract

Microbial keratitis can arise from penetrating injuries to the cornea. Corneal trauma promotes bacterial attachment and biofilm growth, which decrease the effectiveness of antimicrobials against microbial keratitis. Improved therapeutic efficacy can be achieved by reducing microbial burden prior to antimicrobial therapy. This paper assesses a highly-branched poly(N-isopropyl acrylamide) with vancomycin end groups (HB-PNIPAM-van), for reducing bacterial attachment and biofilm formation. The polymer lacked antimicrobial activity against Staphylococcus aureus, but significantly inhibited biofilm formation (p = 0.0008) on plastic. Furthermore, pre-incubation of S. aureus cells with HB-PNIPAM-van reduced cell attachment by 50% and application of HB-PNIPAM-van to infected ex vivo rabbit corneas caused a 1-log reduction in bacterial recovery, compared to controls (p = 0.002). In conclusion, HB-PNIPAM-van may be a useful adjunct to antimicrobial therapy in the treatment of corneal infections.

Graphical abstract: Antibiotic functionalised polymers reduce bacterial biofilm and bioburden in a simulated infection of the cornea

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Publication details

The article was received on 17 Feb 2018, accepted on 17 May 2018 and first published on 04 Jun 2018


Article type: Paper
DOI: 10.1039/C8BM00201K
Citation: Biomater. Sci., 2018,6, 2101-2109
  • Open access: Creative Commons BY license
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    Antibiotic functionalised polymers reduce bacterial biofilm and bioburden in a simulated infection of the cornea

    N. Doroshenko, S. Rimmer, R. Hoskins, P. Garg, T. Swift, H. L. M. Spencer, R. M. Lord, M. Katsikogianni, D. Pownall, S. MacNeil, C. W. I. Douglas and J. Shepherd, Biomater. Sci., 2018, 6, 2101
    DOI: 10.1039/C8BM00201K

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