Jump to main content
Jump to site search

Issue 5, 2018
Previous Article Next Article

Gellan Gum-based luminal fillers for peripheral nerve regeneration: an in vivo study in the rat sciatic nerve repair model

Author affiliations

Abstract

Peripheral nerve injuries (PNI) resulting in a gap to be bridged between the transected nerve ends are commonly reconstructed with autologous nerve tissue, but there is a need for valuable alternatives. This experimental work considers the innovative use of the biomaterial Gellan Gum (GG) as a luminal filler for nerve guidance channels made from chitosan with a 5% degree of acetylation. The engineered constructs should remodel the structural support given to regenerating axons by the so-called bands of Büngner. Four different GG formulations were produced by combining varying amounts of High-Acyl GG (HA-GG) and Methacrylated GG (MA-GG). The effective porosity of the freeze-dried networks was analysed by SEM and micro-CT 3D reconstructions, while the degradation and swelling abilities were characterized in vitro for up to 30 days. The metabolic activity and viability of immortalized Schwann cells seeded onto the freeze-dried networks were also evaluated. Finally, the developed hydrogel formulations were freeze-dried within the chitosan nerve guides and implanted in a 10 mm rat sciatic nerve defect. Functional and histomorphological analyses after 3, 6, and 12 weeks in vivo revealed that although it did not result in improved nerve regeneration, the NGC25:75 formulations could provide a basis for further development of GG scaffolds as luminal fillers for hollow nerve guidance channels.

Graphical abstract: Gellan Gum-based luminal fillers for peripheral nerve regeneration: an in vivo study in the rat sciatic nerve repair model

Back to tab navigation

Supplementary files

Article information


Submitted
27 Nov 2017
Accepted
04 Feb 2018
First published
05 Feb 2018

Biomater. Sci., 2018,6, 1059-1075
Article type
Paper

Gellan Gum-based luminal fillers for peripheral nerve regeneration: an in vivo study in the rat sciatic nerve repair model

C. R. Carvalho, S. Wrobel, C. Meyer, C. Brandenberger, I. F. Cengiz, R. López-Cebral, J. Silva-Correia, G. Ronchi, R. L. Reis, C. Grothe, J. M. Oliveira and K. Haastert-Talini, Biomater. Sci., 2018, 6, 1059
DOI: 10.1039/C7BM01101F

Social activity

Search articles by author

Spotlight

Advertisements