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Synthesis of PB@FePt hybrid nanoparticles with peroxidase-mimicking activity for colorimetric determination of hydrogen peroxide in living cells

Abstract

Rapid determination of H2O2 have been required among series of areas, such as bioanalysis, food safety and clinical diagnosis. In this work, a novel colorimetric sensing platform, based on Prussian blue nanocubes supported FePt (PB@FePt HNPs), for rapid and sensitive determination of H2O2 was constructed. The obtained hybrid nanoparticles exhibited efficient peroxidase-mimicking activities verified by the oxidation reaction of 4,4'-Bi-2,6-xylidine accompanied with H2O2. Under optimized conditions, the linear range for H2O2 determination was 20 μM to 800 μM. Moreover, a relatively low detection limit was acquired, which was reckoned as 8.27 μM. The principle of these peroxidase-mimicking reactions was investigated by fluorometric spectra method with terephthalic acid as indicator. Furthermore, the colorimetric sensing platform was successfully applied to determinate intracellular H2O2 in human breast adenocarcinoma cells (MCF-7), which indicated that as-constructed PB@FePt-TMB-H2O2 system provided a potential approach for H2O2 detection in living cells. Keywords: Colorimetric; Peroxidase-mimicking; PB@FePt, Intracellular H2O2, cancer cell.

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Publication details

The article was received on 25 Nov 2018, accepted on 21 Dec 2018 and first published on 02 Jan 2019


Article type: Paper
DOI: 10.1039/C8AY02567C
Citation: Anal. Methods, 2018, Accepted Manuscript
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    Synthesis of PB@FePt hybrid nanoparticles with peroxidase-mimicking activity for colorimetric determination of hydrogen peroxide in living cells

    Z. Hu, Z. Dai, X. hu, K. chen, C. gao, X. Zheng and Y. Yu, Anal. Methods, 2018, Accepted Manuscript , DOI: 10.1039/C8AY02567C

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