Jump to main content
Jump to site search
PLANNED MAINTENANCE Close the message box

Scheduled maintenance work on Wednesday 27th March 2019 from 11:00 AM to 1:00 PM (GMT).

During this time our website performance may be temporarily affected. We apologise for any inconvenience this might cause and thank you for your patience.


Issue 5, 2019
Previous Article Next Article

Synthesis of PB@FePt hybrid nanoparticles with peroxidase-mimicking activity for colorimetric determination of hydrogen peroxide in living cells

Author affiliations

Abstract

The rapid determination of H2O2 is important in a series of areas, such as bioanalysis, food safety and clinical diagnosis. Here, we describe a novel colorimetric sensing platform, based on Prussian blue nanocubes supporting FePt (PB@FePt HNPs), for the rapid and sensitive determination of H2O2. The hybrid nanoparticles exhibited efficient peroxidase-mimicking activities that were verified by the oxidation reaction of 4,4′-bis-2,6-xylidine accompanied with H2O2. Under optimized conditions, the linear range for H2O2 determination was 20 μM to 800 μM. A relatively low detection limit was acquired reaching 8.27 μM. The principle of these peroxidase-mimicking activities was investigated by the fluorometric spectroscopy method with terephthalic acid as the indicator. Furthermore, the colorimetric sensing platform was successfully utilized to determine the intracellular H2O2 in human breast adenocarcinoma (MCF-7) cells. This indicated that the as-constructed PB@FePt-TMB-H2O2 system provided a potential approach for H2O2 detection in living cells.

Graphical abstract: Synthesis of PB@FePt hybrid nanoparticles with peroxidase-mimicking activity for colorimetric determination of hydrogen peroxide in living cells

Back to tab navigation

Supplementary files

Publication details

The article was received on 25 Nov 2018, accepted on 21 Dec 2018 and first published on 02 Jan 2019


Article type: Paper
DOI: 10.1039/C8AY02567C
Citation: Anal. Methods, 2019,11, 677-683

  •   Request permissions

    Synthesis of PB@FePt hybrid nanoparticles with peroxidase-mimicking activity for colorimetric determination of hydrogen peroxide in living cells

    Z. Hu, Z. Dai, X. Hu, K. Chen, C. Gao, X. Zheng and Y. Yu, Anal. Methods, 2019, 11, 677
    DOI: 10.1039/C8AY02567C

Search articles by author

Spotlight

Advertisements