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Duplex-specific nuclease mediated target recycling amplification for fluorescence detection of microRNA

Abstract

Isothermal amplification techniques for nucleic acid detection have drawn increasing interests in recently due to the simplicity and low-cost of instrument. We have developed a novel duplex-specific nuclease (DSN) mediated target recycling amplification strategy for microRNA detection. The method utilized different adsorption ability between single-stranded DNA and double-strand DNA of graphene oxide (GO) to achieve fluorescence recovery. DSN was employed to mediate target recycling amplification so desirable sensitivity for miR-21 detection was achieved. The developed method also demonstrated excellent selectivity and applicability for analysis of samples in complex biological media. The application of the developed method for analysis of miR-21 level in three different cell lines demonstrated its potential for real sample analysis.

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Publication details

The article was received on 17 Oct 2018, accepted on 26 Nov 2018 and first published on 30 Nov 2018


Article type: Paper
DOI: 10.1039/C8AY02265H
Citation: Anal. Methods, 2018, Accepted Manuscript
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    Duplex-specific nuclease mediated target recycling amplification for fluorescence detection of microRNA

    L. Tan, L. Xu, J. Liu, L. Tang, H. Tang and R. Yu, Anal. Methods, 2018, Accepted Manuscript , DOI: 10.1039/C8AY02265H

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