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Issue 48, 2018
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A dual signal amplification method for miR-204 assay by combining chimeric molecular beacon with double-stranded nuclease

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Abstract

Identifying microRNAs (miRNA) as biomarkers has attracted much attention owing to their key roles in early disease diagnosis and assessing the prognosis and monitoring the outcome of therapy. However, the rapid and efficient detection of microRNA levels remains challenging. By combining the specificity targeting of chimeric molecular beacons with the signal amplification ability of double-stranded nuclease, we now report a rapid, sensitive, and selective miRNA204 assay. This assay can detect miR-204 at concentrations as low as 50 pM with a linear range of 50 pM to 10 nM. This method also shows the ability to discriminate target sequences containing mismatched bases. This could be a promising tool for selective analysis of miRNAs in vitro.

Graphical abstract: A dual signal amplification method for miR-204 assay by combining chimeric molecular beacon with double-stranded nuclease

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Publication details

The article was received on 01 Oct 2018, accepted on 12 Nov 2018 and first published on 21 Nov 2018


Article type: Paper
DOI: 10.1039/C8AY02147C
Citation: Anal. Methods, 2018,10, 5834-5841
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    A dual signal amplification method for miR-204 assay by combining chimeric molecular beacon with double-stranded nuclease

    H. Liu, J. Fan, W. Liu, C. Tong, Z. Xie, R. Deng and X. Long, Anal. Methods, 2018, 10, 5834
    DOI: 10.1039/C8AY02147C

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